Cairo University

MTPR Journal

 

Nano-Eyes: 4D Atomic Force Microscopy (4DAFM) of the Effect of Suicidal Gene Nanoparticles on Live Cancer Cells.


Hosam Gharib Abdelhady
Associate Professor (Biophysics & Surface Analysis), Department of Pharmaceutics and Pharmaceutical

Technology, College of Pharmacy, Taibah University, Almadinah Almunawarrah,
Saudi Arabia.

Vol./Issue: 14 , id: 94

The real time understanding of the suicidal gene effects on the cytoskeletal and nano-mechanical behaviors of cancer cells may provide new, effective ways in cancer gene therapy. Here, 4DAFM was applied to monitor the effect of targeted suicidal gen-nanoparticles on the morphological and nanomechanical properties of individual, dividing cancer cells in their environment. The mechanisms of forming the suicidal gene nanoparticles were also seen in 4D. Left; The transfection of single plasmid into a cancer cell, Right; the transfection of single plasmid-nanoparticles reaching the nucleous and kill the cancer cell.