Cairo University

MTPR Journal

 

BIODISTRIBUTION AND TOXICITY ASSESSMENT OF COPPER NANOPARTICLES IN THE RAT BRAIN

2019-04-21
Heba M Fahmy*,Omnia A Ali, Asmaa A Hassan, Faten A Mahmoud, Fatma M Abdelrahman, MariamMAbu El-fotouh
Biophysics Department, Faculty of Science, Cairo University,Egypt
Vol./Issue: 19 , id: 283

The increase in the usage of copper nanoparticles (CuNPs) in the industrial and medical fields has raised concerns about their possible adverse effects. The present study aims to investigate the potential adverse effects of CuNPs on the brain of adult male Wistar rats through the estimation of some oxidative stress parameters and acetylcholinesterase (AChE) activity.CuNPs were prepared and characterized using different techniques: Dynamic Light Scattering, XRay Diffraction, Transmission and Scanning Electron Microscopy, Fourier transform Infrared Spectroscopy, in addition to Energy Dispersive X-ray Spectroscopy. Rats were divided into two groups: CuNPs-treated group (IV injected with 15 mg/kg ˷ 13 nm CuNPs for 2 successive days) and a control group (injected with saline). Rats of the 2 groups were decapitated simultaneously after 48 hours of the last injection. The Cu content in different brain areas was analyzed using inductively coupled plasma mass spectrometry. Moreover, the effect of CuNPs on brain edema was evaluated.The behavior of rats in an open-field was also examined 24 hours post the last injection.Significant increases of Cu content in the cortex, cerebellum, striatum, thalamus and hippocampus was found. Moreover, CuNPs lead to the induction of oxidative stress condition in the thalamus, hypothamaus and medulla. In addition, CuNPs induced significant increases in AChE activity in the medulla, hippocampus, striatum besides midbrain. CuNPs-injected rats showed alsodecreased exploratory behaviour.The results obtained in the present study point to the importance of toxicity assessments in evaluating the efficiency of CuNPs for the safe implementation in different applications.