Cairo University

MTPR Journal

 

Evaluation of the Cytotoxicity of Thymoquinone-Encapsulated Dppc Liposome for the Inhibition of Prostate and Laryngeal Cancer Cells

2019-04-21
Heba Mohamed Fahmy, Nada Reda Abd Elkader, Taiseer Mohamed Abd Eldaim, Ayaat Mahmoud Mosleh, Omnia Eid Ali, Latifa Helmy Ahmed Biophysics Department, Faculty of Science, Cairo University
A great challenge that we affront nowadays is to treat cancer cells
without affecting healthy cells. In the present work for the first time,
the cytotoxicity effect of free Thymoquinone (TQ) and TQencapsulated
DPPC liposome was evaluated on prostate cancer cells
PC-3 and larynx cancer cells HEP-2 for 24 and 72 hrs, respectively,
using MTT assay. TQ (the main active gradient of Nigella sativa) was
chosen because of its well-known antioxidant, anti-inflammatory and
anticancer activities in vitro. DPPC liposome was prepared by the thin
film hydration method and its encapsulation efficiency for TQ
exceeded 90%. The TQ-encapsulated DPPC liposome was
characterized by FTIR, UV-Vis spectrometer, particle size, DSC in
addition to zeta potential. From the present results, it was found that
treatment of PC-3 and HEP-2 cells with free TQ for 24 and 72 hrs at
37°C resulted in at least 70-85 % inhibition of cellular proliferation
without affecting their viability.On the other hand, TQ- encapsulated
DPPC liposome exhibited similar inhibitory effect only at 10-fold higher
dose. It is recommended to develop liposome formulations with
improved targeting ability to further improve the efficacy of TQ in vivo.

Vol./Issue: 19 , id: 282