Cold atmospheric plasma (CAP) has many promising applications in biomedical engineering, dentistry and oncology. This study investigates the effect of CAP on 1,2-dipalmitoyl-Sn-glycerol-3- phosphocholine (DPPC) liposomes prepared by the thin film hydration method which are used as a model for lipid bilayer membrane. DPPC liposomes were exposed to cold plasma 2, 3 and 5 minutes. The effects of cold plasma on DPPC characterization parameters such as size, charge, FTIR absorption spectrum, UV-visible spectrum and phase transition temperatures were investigated. Exposure of DPPC liposomes to CAP led to an increment in the size and stability of liposomes by increasing the exposure time. 2-min CAP exposure was found to increase liposomal size through particle aggregation, whereas, 3 and 5 min exposure increases the liposome size via adsorption of negative species emerging from CAP on their surfaces. As depicted from differential scanning calorimetry (DSC) results, the electrostatic interaction between the CAP species and phospholipids acyl groups of DPPC resulted in the change of DPPC conformation. The hydrocarbon chains of lipid molecules arranged more closer, especially with increasing the exposure time. Thus, it can be concluded that CAP alters the physical and chemical characteristics of DPPC liposomes.
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