Cairo University

MTPR Journal

 

Study The Genetoxicity Of Gold Magneto Nanoparticles In Liver Cancer Cells Via Laser Induced Plasma Spectroscopy

2016-12-17
Ola S. Ahmed1 , H. Imam 2, Abdel-Rahman Zekri3Mona B. Mohamed2, Hussein M Khaled1 Mahmoud H Abdel-kader2,4
1,3National Cancer institute, Cairo University, Egypt.
2National institute of laser enhanced science (NILES), Cairo University, Egypt
4German University in Cairo, Egypt

Vol./Issue: 16 , id: 219

Laser-induced breakdown spectroscopy technique (LIBS) used in the study the effect of gold magneto nanoparticles on the liver cancer cells (HepG2) by examining the variation in genotoxicity and cytotoxicity.HepG2 cell line (106 cells) was cultured and expanded, after which, they were incubated with gold magneto nanoparticles at concentrations 10,25, 50 μg/ml at 12 hours. The effect of this nanoparticles in cell apoptosis as well as in autophagy were evaluated by both flow cytometry and acridine orange.Annexin V/propidium iodide double staining for evaluation of apoptosis in the treated cells at concentration of nanocomposite 10, 25, 50μg/ml showed increase in the apoptotic presented from 11.25% ± 3.1% to 28% ± 2.04%,39% ± 3.6%, 60% ± 4.3% respectively.The gene expression of apoptosis pathway was also investigated by apoptosis specific PCR array assay.The genes most high significantly in HepG2 cells were TNF, BIRC3 and NFKB1 fold regulation ratio of 158.03- fold and 149.2- fold. These genes expression profiles related to stress and toxicity pathways.LIBS results shows that increasing iron oxide nanoparticles concentration is associated with increasing calcium concentration in the biological cells. This effect might be due to increasing the probability of a calcium influx in single cells due to higher surface iron oxide loading and the degree of cell spreading.An increase in the calcium content in the cells have a role in program cell death was prove it by profile apoptosis PCR array.Finally we have demonstrated successfully that LIBS can be an effective technique for the detection of magnetic nanoparticles concentrations present in liver cancer cells (HepG2 cells) and study the effect of these particle through investigation variation in genotoxicity and cytotoxicity.