Silver nanoparticles have attracted much attention in antibacterial coatings, biological sensors, textiles, and biomedical devices because of their size-dependent properties. Pulsed laser ablation in liquids confine the movement of the resultant plasma plume which can greatly influence the kinetic properties. This causes distinctly different environments of the condensing phase formation from that of laser ablation of solids in vacuum or diluted gas. In this study, silver nanoparticles were prepared by two different techniques namely liquid phase pulsed laser ablation (LP-PLA) and chemical reduction. In the LP-PLA, IR and UV lasers types were used to ablate silver rods in distilled water. The IR laser was Nd:YAG with = 1064 nm, pulse duration = 6 ns, and 110 mJ laser energy. The UV type was nitrogen laser with = 337 nm, pulse duration = 15 ns, and 375 mJ laser energy. In the chemical reduction, silver sulphate, sodium borohydride, and tri-sodium citrate were used to prepare the silver nanoparticles. The fabricated nanoparticles were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD) and energy dispersive X-ray (EDX) microanalysis in order to analyze the final size and composition of nanoparticles. For sample prepared by Nd:YAG laser, the measured average size was found to be ~9.9 nm and sample prepared by chemical reduction showed average size of ~13.9 nm. The results show about spherical shape for samples prepared by Nd:YAG laser and chemical reduction while needle shape produced by the nitrogen laser. These silver nanoparticles will be applied to cotton fabrics for studying the dyeing behavior of the treated fabrics, such as color strength measurement and color fastness measurement.