ortant role in various applications such as material processing, and biomedical applications. Atmospheric Plasma jet (APJ) is one of the most widely used methods for generating non thermal atmospheric plasma. The jet plume consists of various groups of highly reactive chemical agents such as reactive oxygen species (ROS) including oxygen atoms O, hydroxyl group OH, hydrogen peroxide H2O2 and Ozone O3. The new field of plasma medicine is rapidly advancing toward the development of new medical therapies such as skin diseases, disinfection of dental cavities, dermatology, cancer treatment, and many others. The ROS generated by plasma jet can penetrate the cells and might induce high levels of DNA damage, resulting in apoptosis. The main goal of the paper is design and characterize atmospheric plasma jet APJ with high electron density and high reactive oxygen species (ROS) that targeting different types of cancer cell line (in-vitro) and other biomedical applications, through ROS-reaction mechanisms which can enhance the role of cold plasma cancer and medical therapy in Egypt. A schematic diagram and real photo of a dielectric barrier discharge plasma APJ is shown in figure 1a,b that is designed in our Lab.